Etiological agents in patients with acute diarrheal disease detected by PCR test in children from 0 to 14 years old in Hospital Metropolitano of Quito
Keywords:
etiology, gastrointestinal infections, PCR gastrointestinal panelAbstract
Objective: Determine the prevalence of gastrointestinal infections in children from 0 to 14 years old at Hospital Metropolitano of Quito from August 2017 to May 2018.
Methods: A cross sectional study was carried out to determine the prevalence of gastrointestinal infections in children from 0 to 14 years old at Hospital Metropolitano of Quito. There were 58 patients with gastrointestinal infection from August 2017 to May 2018. We used the results from PCR gastrointestinal panel to determine the etiology of the disease.
Results: We studied 58 patients with gastrointestinal infection between 0 to 14 years old. We found 79% of bacterial and 21% viral etiology. The most frequent agent was Clostridium difficile (15%) followed by enteropathogenic E. coli (14%), enteroagregative E. coli (12%). The most important virus was norovirus followed by rotavirus, and Giardia lamblia as a parasite. The most frequent coinfections were Clostridium difficile-Campylobacter, Clostridium difficile-enteropathogenic E. coli, enteropathogenic E. coli-enterotoxigenic E. coli. The months of the year where the greatest number of infections occurred were April and May.
Conclusions: The etiology of gastrointestinal infections is a very important issue to study and manage because of its high incidence at the pediatric population, so a timely diagnosis and comprehensive management must be made.
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References
Sandra M, Amaral C, Estevam GK, Penatti M, Lafontaine R, Carlos I, et al. The prevalence of norovirus , astrovirus and adenovirus infections among hospitalised children with acute gastroenteritis in Porto Velho , state of Rondônia , western Brazilian Amazon. Mem Inst Oswaldo Cruz, Rio de Janeiro 2015;110(April):215–221.
Roig CG, Larre N, Pastene H. Epidemiological and clinical characteristics of acute gastroenteritis according to their etiology (rotavirus or other) in children younger than 5 years old treated in a private institution in the City of Buenos Aires. Archivos Argentinos de Pediatría 2013;111(3):218–223.
Organización Mundial de la Salud [Internet]. Enfermedades diarreicas. 2017. Cited: 2018/Mayo/07. Avaible from: http://www.who.int/es/newsroom/ fact-sheets/detail/diarrhoeal-disease
Zhu XH, Tian L, Cheng ZJ, Liu WY, Li S, Yu WT, et al. Viral and bacterial etiology of acute diarrhea among children under 5 years of age in Wuhan , China. Chinese Medical Journal 2016;129(16):1939–1944.
Balkan ÇE, Çelebi D. Acute gastroenterıtıs agents under 5 years old age chıldren. Medical and Engineering Sciences 2017;8–11.
Puruncajas J. Identificación etiológica de la enfermedad diarreica aguda (EDA) en niños de dos meses a cinco años de edad en el servicio de emergencia de la Novaclínica Santa Cecilia en el período de mayo a diciembre del 2013, “Disertación”. Repositorio PUCE. Puruncajas J; 2013. 37-51.
Vollrath DV. Artículo de revisión. Biología molecular en el diagnóstico clínico, aporte del desarrollo de las técnicas de biología molecular en la medicina de hoy. Revista electrónica científica y académica de Clínica Alemana. 2015;143–148.
Farfán M, Piemonte P, Labra Y, Henríquez J, Candia E, Pablo J. Panel Filmarray GI ® en la detección de patógenos entéricos en deposiciones: experiencia preliminar. Revista chilena de infectología 2016;99(1):89–91.
Balsalobre-Arenas L. Diagnóstico rápido de las infecciones del tracto gastrointestinal por parásitos, virus y bacterias. Enfermedades Infecciosas y Microbiología Clínica 2017;35(6):367–376.
PAHO [Internet]. Enfermedades diarreicas. 2017. Cited: 2018/Mayo/10. Avaible from: https://www.paho.org/ecu/index.php?option=com_content&view=article&id=1878:1-7-millones-de-ninos-menores-decinco-
anos-fallecen-anualmente-por-contaminacion-ambiental-segunla-oms&Itemid=360
Montenegro S, Pineda S, Enríquez I, Enríquez N, Rivera N. Detección de norovirus en niños con diarrea adquirida en la comunidad o nosocomial en el Hospital Guillermo Grant Benavente de Concepción, Chile. Revista Chilena de Infectología 2014;31(3):298–304.
Edwards KM, Staat MA, Weinberg GA, Hall CB, Chappell J, Ph D, et al. Norovirus and medically attended gastroenteritis in U.S. Children. The New England Journal of Medicine 2013;1121–1130.
Naranjo A. Caracterización de los serotipos de rotavirus en Ecuador. Virology, 2008
Hernández C, Aguilera A. Situación de las enfermedades gastrointestinales en México. Enfermedades Infecciosas y Microbiología. 2011;31(4):137–151.
Benito AG, Barba PG, Alonso JCR, Hernández PG, Pediatría S De, Virgen H, et al. Infecciones gastrointestinales prevalentes en pediatría. Bol Pediatr. 1998;38:220–241.
El-sayed NM. The impact of intestinal parasitic infections on the health status of children: An Overview. Journal of Pediatric Infectious Diseases 2017;12:209-213.
Thamizhmani R, Purushottaman A, Ramya SP. Intestinal parasitic infestation among paediatric diarrhoea patients attending hospitals in Port Blair, Andaman and Nicobar Islands. Journal of Parasitic Diseases 2016;41:584-589.
Fahmy HM, Deeb HK El, Hussein HM. Giardia duodenalis assemblages in Egyptian children with diarrhea. Eur J Clin Microbiol Infect Dis 2015;34:1573-1581.
Nordgren J. Rotavirus and norovirus in children with severe diarrhea in Burkina Faso before rotavirus vaccine introduction. Journal of Medical Virology 2018:1:0-3.
Kim SY, Kim H, Shin EH, Eun BW, Ahn YM, Song MO. Etiology and clinical features of acute bacterial gastroenteritis in children. Pediatr Infect Vaccine 2017;24(2):95-101.
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